Bioanalysis of non-water based industrial media

Microbially contaminated fuel poses a serious threat to aviation. Condensation or inadequate storage can lead to the formation of water in the tanks. The resulting water phase provides an ideal environment for the growth of microbes such as the fungus Hormoconis resinae (also known as kerosene fungus) and bacteria. These microorganisms can cause corrosion and form biofilms, which can in turn lead to leaks and blockages in fuel lines, filters, and pumps, resulting in performance losses or even engine failure.

To detect harmful contaminants from microorganisms in a timely manner and to implement appropriate countermeasures, regular monitoring of the micro-biological contamination of fuel is essential. There are four test methods that allow for a quantitative or semi-quantitative assessment of contamination. The first method is based on the cultivation of microorganisms, whereby the total number of viable colony-forming units can be counted by laboratory personnel. The second method involves testing for adenosine triphosphate (ATP), a biochemical compound produced by all living organisms. Another test method is based on an immunoassay to examine the sample. This involves testing reagents for biomarkers specific to the microorganisms that are associated with the contamination. The fourth test method is based on the molecular determination of microbial genome, gene activation, proteins, or metabolic activity. qPCR is one of the typical molecular analysis techniques known for its specificity and quantitative results. The IATA (International Air Transport Association) is currently supporting a program to develop new recommendations for testing methods. This is due to the need, resulting from airline experience, to re-evaluate technologies already used in commercial test kits as well as new testing procedures.

Innovative Solutions: On-Site Investigations

At Fraunhofer IMM, we have developed a miniaturized platform that enables fully automated processing and analysis of kerosene samples using qPCR analysis. The system allows for the direct detection of microorganisms in up to one liter of kerosene within just one hour. Automation minimizes human influences on the results. Additionally, combined with miniaturization, testing costs are significantly reduced as no trained personnel or laboratory environment are needed. This enables us to deliver high-quality results in a short time at reasonable costs. The system consists of various functional modules that represent individual process steps and can each be used as standalone devices.

Performance of Our Technology:

Our Offer to Our Customers

Based on your individual needs, we develop tailored solutions that go far beyond the current state of the art. With a variety of manufacturing methods directly on site, including cleanroom, mechanical workshop, electronics workshop, and software development, we are well-equipped to meet your requirements and realize innovative solutions. Our interdisciplinary team brings unique expertise in microfluidic system development and has extensive know-how in the manufacturing and validation of microfluidic analysis systems. This guarantees both efficiency in development and a smooth implementation of your projects.

Our service offerings include:

• Feasibility studies: Analysis and optimization of complex analytical processes.
• Implementation of your application idea: From concept to proof of function.
• Development of microfluidic individual modules: Tailored solutions for your specific needs.
• Construction of automated demonstrators: Fully functional systems for on-site and in-line analytics.
• Validation of the overall system: From the first prototype to the pre-production series.

Whether you want to bring stationary detection to the site of action or establish new analyses that were previously not possible, together we will find the perfect solution for your challenges.